They make a rough landing on Delfino Airstrip, as a large amount of Graffiti in the shape of Mario's head is blocking the runway. Peach, however, sees a Mario-shaped shadow figure jumping around in the background. Mario is only interested in the food there, while Toadsworth is only interested in the rest he will get when they land on the island. A TV screen on the Toad Express is showing an advertisement of Isle Delfino.
The game starts off in the Toad Express, where Mario, Princess Peach, a few Toads, and Toadsworth are flying out to Isle Delfino for a vacation. Mario faces the first of many Polluted Piranha Plants.